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KMID : 1147820230120010007
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Journal of Naturopathy
2023 Volume.12 No. 1 p.7 ~ p.15
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Isolation and Structural Identification of Antioxidant Substances from Ethyl Acetate Extract of Conyza canadensis
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Song Hyun-Sook
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Abstract
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Background: As a result of analyzing the components of wild Conyza canadensis, it contains physiologically active ingredients, so it is necessary to identify the compound.
Purposes: It was to study the compound's molecular structure; a previous study showed that C. canadensis contains antioxidant substances.
Methods: The ultrasonic pulverized lysate of C. canadensis stem and leaves was first extracted with 90% methanol and then five organic solvents. Next, the extracts was fractionated by HPLC, LC/MS chromatography, and NMR analyzers identified the molecular structure.
Results: 100 g of dry C. canadensis was sonicated in 90% methanol and concentrated under reduced pressure to 11.96 g of a crude extract. Then, this crude was extracted with five types of solvents to obtain 123.8 mg of n-hexane, 448.2 mg of dichloromethane, 1047.7 mg of ethyl acetate (EA), 2563.8 mg of butanol, and 7.04 g of water. The EA extracts were fractionated by LC-MS and then re-fractionated to obtain F1 to F20. Next, the F15 was further fractionated to obtain nine fine fractions. Finally, the F17 fraction was re-fractionated to obtain ten fine fractions. As a result of LC-MS and NMR spectrometer analysis of the F15-7, the structure of this compound was confirmed as 3,5-di-caffeoylquinic acid. As a result of examining the structures of the F17-4 and F17-5 fractions, Quercetin-3-o-β-galactose was identified. In addition, the form of the F17-10 was confirmed to be 1,3,4-tri-caffeoylquinic acid.
Conclusions: This study demonstrated that C. canadensis contained phenolic antioxidants, and its utilization may be expected.
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KEYWORD
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acetyl acetate, NMR((nuclear magnetic resonance), C. canadensis, antioxidant substance,ethyl acetate, NMR(nuclear magnetic resonance) spectrometer
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